Different from other methods, Ward’s algorithm tries to find as homogeneous groups as possible. This region of IR spectrum is called as finger print region of the molecule. Both tissues are capable to store triglycerides . Applications of IR spectroscopic techniques allow identifying molecular changes due to obesity, to understand the molecular mechanism of the disease, to identify specific spectral biomarkers that can be used in diagnosis. Here in this chapter, we will discuss the mid and near IR spectroscopy in obesity research. Indeed, as the grade of steatosis was lowered, a decrease in glycogen content with a concomitant increase in lipids was observed in comparison to normal liver. Biological applications. In current chapter, the applications of IR (mid and near), spectroscopic and microspectroscopic techniques in obesity research will be discussed in detail. IR spectrum of the test sample to be determined is compared with the standard compound. The other factors while choosing solvent are that it should be as nonpolar as possible to minimize solute–solvent interactions; and it should not strongly absorb IR radiation. Nonalcoholic fatty liver disease (NAFLD) is a frequent lesion associated with obesity, diabetes, and the metabolic syndrome.  studied the effect of dietary fatty acid composition on lipolysis. Barbara is the author of three other books published by John Wiley and Sons, Ltd, namely Modern Infrared Spectroscopy and Biological Applications of Infrared Spectroscopy, both in the ACOL series of open learning texts, and Polymer Analysis in this current AnTS series of texts. The disk thickness is important; thin disks are fragile and difficult to handle, while thick disks transmit too little radiation. In this section, the basic ideas and definitions associated with IR spectroscopy and microscopy will be described. White adipose tissue has been organized in different anatomical location as visceral white adipose tissue (VAT) and subcutaneous white adipose tissue (SCAT). Spectroscopy - Spectroscopy - Applications: Spectroscopy is used as a tool for studying the structures of atoms and molecules. Such materials include Diamond, zinc selenide (ZnSe), germanium (Ge), silicon (Si), and thallium/iodide. The position of the spectral bands allows us to do band assignments. Besides aqueous samples, dry samples, homogeneous soft tissues, or hard tissues can also be studied with the ATR–IR spectroscopy by directly placing a small portion of the sample on the crystal and then applying the same pressure to all the samples. A representative baseline corrected normalized FTIR spectrum of a human adipose tissue in the 4000–750 cm−1 region. In the other study of the same group, lipid profiles in terms of the content and structure of skeletal muscle and adipose tissues were determined to understand better the characteristics of juvenile-onset spontaneous obesity without high fat diet induction. Spectral differences indicated differences in lipid structure and content of BFMI lines, which may originate from different insulin sensitivity levels of the lines. Infrared spectroscopy is a simple and reliable technique widely used in both organic and inorganic chemistry, in research and industry. The products of VAT are driven to the liver via portal vein system, affecting its function. Finally, tissue samples are thaw-mounted on IR-transparent CaF2 or BaF2 windows for IR mapping and data processing . A spectroscopic technique used to observe vibrational, rotational, and other low-frequency [modes in a system. 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